For those who don't know, yes, this is a serious consideration as Microsoft have been discussing bringing the show back for a while.
If it does, he said, it will likely be with an entirely new cast and characters--and he can't see himself reprising his role as Sylar, even if asked.
"I don't think that I would ever go back to that, personally," Quinto told the audience. "I had an incredible time and it defined so much of my journey as an actor from that point forward. I'm very grateful for it; I had the best time working on that show; I loved that character, I loved the people that I got to work with. But it's that feeling taht it's a chapter that is closed a little bit, you know? I have moved on to other stuff and I'm hungry to continue to do that."
That's a familiar tune; his "I'm eager to move on" speech had briefly convinced some members of the entertainment press that he might be ready to leave the Star Trek franchise as well following Star Trek Into Darkness (that impression has since been dispelled).
Quinto is expected to appear in a third, as-yet-untitled Star Trek film, along with the rest of the current cast of the J.J. Abrams-directed reboot. Abrams will not return, opting instead to move on to Star Wars, and it's anyone's guess which, if any, of the actors will remain on board after the third film.