Every piece of promotional material for Avengers: Infinity War has featured the Infinity Stones in the same arrangement when in Thanos' possession.
A Marvel fan took his detective work to Photoshop to work up an image with each look at the Infinity Gauntlet which has been revealed by Marvel Studios. In each of the photos, the Infinity Stones are assembling in the same order in the Infinity Gauntlet. Though the first trailer for Avengers: Infinity War merely featured two stones being in Thanos' possession, their alignment matches that of the other promo materials.
Check out the photo below, which uses the D23 art, San Diego Comic Con poster, a cast and crew hat, a trailer screenshot, and an image of Josh Brolin holding the Gauntlet to show the Infinity Stones' alignment.
While it is unknown whether or not Thanos will assemble all six Infinity Stones during Avengers: Infinity War, the promotional material seems to indicate he will be seen holding a fully formed Gauntlet at some point -- with the Infinity Stones being in the exact order above. What it means for the Avengers is also unknown, but it's probably not a good thing.
An unprecedented cinematic journey ten years in the making and spanning the entire Marvel Cinematic Universe, Marvel Studios’ Avengers: Infinity War brings to the screen the ultimate, deadliest showdown of all time. The Avengers and their Super Hero allies must be willing to sacrifice all in an attempt to defeat the powerful Thanos before his blitz of devastation and ruin puts an end to the universe.
Anthony and Joe Russo direct the film, which is produced by Kevin Feige. Louis D’Esposito, Victoria Alonso, Michael Grillo and Stan Lee are the executive producers. Christopher Markus & Stephen McFeely wrote the screenplay. Avengers: Infinity War releases in U.S. theaters on May 4, 2018.